Masuo Kino was born April 2, 1929 in Kahuku Mauka (near N"mole), Hawai'i. His father, Kenkin Kino, was a laborer and independent sugarcane grower for Hakalau Plantation Company. His mother, Makato Inamine Kino, was a housewife who raised five children of which Masuo was the youngest. Kenkin and Makato Kino were immigrants from Okinawa. Masuo helped his father and mother in the sugarcane fields.

Ku‘ulei has been working at the Hawai’i Institute of Marine Biology’s Coral Reef Ecology Lab since 1992 under the direction of Drs. Paul Jokiel and Fenny Cox, as an HIMB faculty member since 2005 and as the Principal Investigator of the Coral Reef Ecology Lab since 2016. Over 100 articles have been published in peer-reviewed journals, published reports, and conference proceedings. She was in the top five most read PeerJ journal articles in 2017.